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01494 563377

Pharmaceutical

A More Versatile C18 Column:

The Aqueous C18 - a single phase solution for multiple Dietary Supplement Applications

By Ty W. Kahlerm Pharmaceutical Innovations Chemist and Rick Lake, Pharmaceutical Market Development Manager

 

  • Extends the analytical range of your C18.
  • Better retention of hydrophilic compounds than on a standard C18.
  • Compatible with 100% aqueous mobile phases.

 

Method development continually ranks in surveys as one of the most challenging and time-consuming issues in HPLC. Choosing the right analytical column is the cornerstone of developing sound methods, but with the number and complexity of columns available on the market today, this can be a difficult process. Evaluating multiple columns quickly becomes expensive and laborious, so it is good practice to begin method development with a versatile column. Conventional alkyl (C18) columns are frequently used, but limited by extremely poor retention of hydrophilic compounds. In comparison, Aqueous C18 phases are more versatile than standard C18 phases, due to much higher hydrophilic retention. In this article we demonstrate the broad utility of Aqueous C18 columns across a range of analytes relevant to dietary supplement testing.

Ideal for Multivitamin Analyses—Easily Retains Water-Soluble Vitamins

Many consumers are augmenting their diets with multivitamins. These supplements usually contain multiple water-soluble vitamins in a variety of chemical forms and concentrations. While water-soluble vitamins can be analyzed by HPLC, obtaining adequate retention of hydrophilic analytes is often problematic. As shown in Figure 1, the Ultra II® Aqueous C18 column provides excellent retention and completely resolves a test mix of seven B vitamins.

Also, due to the column's unique bonding chemistry, 100% aqueous mobile phases can be used to elute highly polar compounds, such as ascorbic acid (vitamin C), which are not retained on a conventional C18 (Figure 2). Organic acids can also be retained, further demonstrating column versatility (Figure 3). The Aqueous C18 phase provides greater retention of hydrophilic compounds than conventional C18 columns, while still supplying the hydrophobic retention expected from a standard C18.

Maximum Versatility—Use the Same Phase for cGMP Pesticide Screens

The biggest challenge facing many dietary supplement laboratories today is becoming compliant with FDA current Good Manufacturing Practices (cGMP) requirements. One of the new hurdles presented by cGMP is implementing a comprehensive screening method for pesticide residues in articles of botanical origin. This testing requires either GC/MS or LC/MS/MS, which is a technology change from traditional HPLC assays. Aqueous C18 columns are ideal for LC/MS/MS screening of pesticides residues, as hundreds of pesticides can be resolved and quantified in the same run (Figure 4). Multiresidue pesticide analysis is simplified by the unique selectivity and excellent resolution of the Ultra Aqueous C18 column, which can retain even difficult polar species.

The Aqueous C18 phase, available on several silicas, is an excellent general purpose phase that performs well for many analyses in today's dietary supplement laboratory. Columns are available in a full range of scalable particle sizes, from 1.9µm UHPLC columns to traditional 5µm HPLC columns. Regardless of the instrumentation used, the unique selectivity and enhanced hydrophilic retention of Aqueous C18 columns makes them more versatile than a standard C18 and ideal for method development.

 

Figure 1: A selective separation of seven B vitamins using a simple buffer:acetonitrile gradient on an Ultra II® Aqueous C18 column.
 PeaksRT (min.)
1. Vitamin B3 2.472
2. Vitamin B6 2.764
3. Vitamin B5 3.804
4. Vitamin B9 4.126
5. Vitamin B12 4.261
6. Vitamin B2 4.329
7. Vitamin B7 4.472
Column

Ultra II® Aqueous C18 (cat.# RE9608565)

Dimensions: 150 mm x 4.6 mm ID
Particle Size: 5 µm
Pore Size: 100 Å
Temp.: 40 °C
Sample
Diluent: water:methanol (70:30)
Conc.: 100 µg/mL each component
Inj. Vol.: 1 µL
Mobile Phase
A: 10mM potassium phosphate (pH 2.5)
B: acetonitrile
Time (min.) %B
0 2
1.5 2
5 75
Flow: 1.5 mL/min.
Max Pressure: 105 bar
Detector UV/Vis @ 210 nm
Instrument Shimadzu UFLCXR

LC_PH0503
Figure 2: Retention of the polar compound ascorbic acid (vitamin C) using a 100% aqueous mobile phase on an Ultra II® Aqueous C18 column.
 PeaksRT (min.)
1. Ascorbic acid (vitamin C) 1.759
Column

Ultra II® Aqueous C18 (cat.# RE9608565)

Dimensions: 150 mm x 4.6 mm ID
Particle Size: 5 µm
Pore Size: 100 Å
Temp.: 40 °C
Sample
Diluent: water
Conc.: 100 µg/mL
Inj. Vol.: 2 µL
Mobile Phase 100% 10mM potassium phosphate (pH 2.5)
Flow: 1.5 mL/min.
Max Pressure: 95 bar
Detector UV/Vis @ 242 nm
Instrument Shimadzu UFLCXR

LC_PH0504
Figure 3: The analysis of organic acids using a 100% aqueous mobile phase and an Ultra II® Aqueous C18 column.
 PeaksRT (min.)
1. Tartaric acid 2.061
2. Quinic acid 2.210
3. Malic acid 2.596
4. Citric Acid 4.177
5. Fumaric Acid 5.853
Column

Ultra II® Aqueous C18 (cat.# RE9608565)

Dimensions: 150 mm x 4.6 mm ID
Particle Size: 5 µm
Pore Size: 100 Å
Temp.: 30 °C
Sample
Diluent: Water
Conc.: 10 µg/mL fumaric acid; 2,000 µg/mL each other acids
Inj. Vol.: 5 µL
Mobile Phase 100% 20mM potassium phosphate (pH 2.5)
Flow: 1.0 mL/min.
Detector UV/Vis @ 226 nm
Instrument Shimadzu UFLCXR

LC_PH0498
Figure 4: Comprehensive LC/MS/MS pesticide screen using an Ultra Aqueous C18 column.

A. Pesticides in positive ion mode. LC_FF0490
See Table 1 for compound identification and MS details.

B. Pesticides in negative ion mode. LC_FF0490A
See Table 2 for compound identification and MS details.

C. Improved retention of difficult pesticides. LC_FF0490B
See Table 1 for compound identification and MS details.
Column: Ultra Aqueous C18
Cat. #:

RE9178312

Dimensions: 100mm x 2.1mm
Particle size: 3µm
Pore size: 100Å
Sample: multicomponent pesticide standard
Inj.: 10µL
Conc.: 1ppb each pesticide
Sample diluent: acetronitrile
Conditions:
Instrument: Shimadzu Prominence® UFLCXR
Mobile phase: A: 10mM NH4OAC in water
B: 10mM NH4OAC in methanol
Time (min.) %B
0.0 20
8.0 90
12.0 100
14.8 100
14.9 20
Flow: 500µL/min.
Temp.: 35°C
Det.: Applied Biosystems 4000 QTRAP® LC/MS/MS system
Ion Source: TurboIonSpray®
A&C: ESI+
B: ESI-
IonSpray Voltage: 5kV (ESI+), -4.2kV (ESI-)
Gas 1: 50psi
Gas 2: 60psi
Source Temp.: 600°C

A. LC_FF0490 B. LC_FF0490A C. LC_FF0490B
Table 1: Peak list for pesticides in positive ion mode.
Compound ID Retention Time
(min)
Transition 1 Transition 2
Acephate 1.27 184.1 -> 143 184.1 -> 143
Acetamiprid 4.44 223.2 -> 126.1 223.2 -> 99.1
Acibenzolar-S-methyl 7.22 211 -> 136 211 -> 140
Alanycarb 7.91 400.1 -> 238.2 400.1 -> 91.1
Aldicarb 4.49 208.2 -> 116.1 208.2 -> 89.1
Aldicarbsulfone 2.03 223.1 -> 86.1 223.1 -> 148
Aldicarbsulfoxide 2.2 207.1 -> 132.1 207.1 -> 89.1
Ametryn 6.96 228.1 -> 186.1 228.1 -> 96
Aminocarb 5.37 209.1 -> 137.1 209.1 -> 152.1
Avermectin B1a 11.2 890.5 -> 305 890.5 -> 145
Avermectin B1b 11.4 876.5 -> 291 876.5 -> 145
Azoxystrobin 6.78 404.1 -> 372.1 404.1 -> 344.1
Benalaxyl 7.78 326.2 -> 148.2 326.2 -> 91.1
Bendiocarb 5.1 224.2 -> 109.2 224.2 -> 167.2
Benfuracarb 8.33 411.2 -> 195.1 411.2 -> 252.1
Benthiavalicarb 6.87 382.1 -> 116 382.1 -> 180.1
Benthiavalicarb-isopropyl 6.87 382.1 -> 196.8 382.1 -> 179.9
Benzoximate 7.98 364 -> 199 364 -> 105
Bitertanol 7.98 338 -> 70 338 -> 269
Boscalid 6.98 343 -> 307 343 -> 140
Bromuconazole (isomer 1) 7.36 378 -> 159.1 378 -> 161
Bromuconazole (isomer 2) 8.05 378.1 -> 159.1 378.1 -> 161
Bufencarb 5.18 222.2 -> 95.1 222.2 -> 71
Bupirimate 7.65 317 -> 166 317 -> 108
Buprofezin 8.53 306.2 -> 201.1 306.2 -> 116.2
Butafenacil 7.08 492.2 -> 331.1 492.2 -> 180.1
Butocarboxim 4.4 191.1 -> 75 191.1 -> 116
Butocarboximsulfoxid 2.1 207 -> 74.9 207 -> 90
Butoxycarboxim 1.95 223.1 -> 106 223.1 -> 166
Carbaryl 5.63 202.1 -> 145 202.1 -> 127
Carbendazim 4.52 192.2 -> 160.2 192.2 -> 132.1
Carbetamide 4.74 237 -> 192 237 -> 118
Carbofuran 5.18 222.2 -> 123.1 222.2 -> 165.2
Carboxine 5.61 236 -> 143 236 -> 87
Carfentrazone-ethyl 7.53 412 -> 346 412 -> 366
Chlorfluazuron 9.18 540 -> 158 540 -> 383
Chloroxuron 7.53 291 -> 72 291 -> 218
Chlorpyrifos 8.35 350 -> 198 350 -> 96.9
Chlorpyrifos-methyl 7.88 324 -> 125.1 322 -> 125.1
Chlortoluron 6.31 213.1 -> 72.2 213.1 -> 46.2
Cinidon-ethyl 8.71 394.1 -> 348.1 394.1 -> 107
Clethodim 5.81 360 -> 164 360 -> 268
Clofentezine 8.27 303 -> 138 303 -> 102
Clomazone 7.04 240.1 -> 125 240.1 -> 89.1
Clothianidin 3.35 250 -> 169.1 250 -> 132
Cyanazine 5.51 241.2 -> 214.2 241.2 -> 104.1
Cyazofamid 7.4 325 -> 108 325 -> 261
Cycluron 6.71 199.1 -> 89.1 199.1 -> 89
Cymoxanil 3.91 199 -> 128 199 -> 111
Cyproconazole (isomer 1) 7.45 292.2 -> 70.2 292.2 -> 125.2
Cyproconazole (isomer 2) 7.44 292.1 -> 70.2 292.1 -> 125.2
Cyprodinil 8.15 226 -> 93 226 -> 77
Cyromazine 2.63 167.2 -> 85.1 167.2 -> 68.2
Demeton-S-methyl 2.9 230.9 -> 89.1 230.9 -> 61
Demeton-S-methyl-sulfon 5.6 262.9 -> 108.9 262.9 -> 169
Desethyl-atrazine 3.9 188.1 -> 146.2 188.1 -> 104.1
Desisopropyl-atrazine 3.2 174.1 -> 104.1 174.1 -> 96.1
Desmedipham 6.23 318 -> 182 318 -> 136
Desmethyl-pirimicarb 5.11 225 -> 72 225 -> 168.1
Diazinon 7.88 305.1 -> 169.2 305.1 -> 97
Dichlorvos 5.36 221 -> 109.1 223.1 -> 109.1
Diclobutrazol 7.58 328 -> 70 328 -> 160
Dicrotophos 3.47 238 -> 112 238 -> 193
Diethofencarb 6.56 268 -> 226 268 -> 180
Difenoconazole (isomer 1) 8.35 406.2 -> 251.1 408.2 -> 253.1
Difenoconazole (isomer 2) 8.35 406.1 -> 251.1 408.1 -> 253.1
Difenoxuron 6.78 287.2 -> 123.2 287.2 -> 72
Diflubenzuron 7.62 311 -> 158.2 311 -> 141.2
Dimethenamide 6.9 276.2 -> 244.1 276.2 -> 168.3
Dimethoate 3.58 230 -> 125 230 -> 199.1
Dimethomorph 7.22 388.2 -> 301.1 388.2 -> 165.2
Dimoxystrobin 7.6 327.1 -> 205 327.1 -> 116
Diniconazole 8.07 326 -> 70 326 -> 159
Dinotefuran 2.02 203.1 -> 114.1 203.1 -> 129
Dioxacarb 3.68 224 -> 123 224 -> 167.1
Diphenylamin 7.03 170.1 -> 93 170.1 -> 92
Disulfoton 6.08 275.1 -> 89 275.1 -> 61
Diuron 6.7 233.1 -> 72 235.1 -> 72.1
Edifenphos 6.66 311 -> 283 328 -> 283
EPN 6.72 324 -> 157.1 324 -> 296
Epoxiconazole 7.65 330 -> 121 330 -> 101
Etaconazole 7.57 328.2 -> 159.1 328.2 -> 123
Ethiofencarb 5.58 226.1 -> 106.9 226.1 -> 164.1
Ethiofencarbsulfoxid 3.48 242 -> 107 242 -> 185.1
Ethion 7.9 385 -> 199 385 -> 171
Ethiprole 6.62 397.3 -> 351 397.3 -> 255.5
Ethofenprox 9.66 394.1 -> 107.1 394.1 -> 177.2
Ethofumesate 6.54 304 -> 121 304 -> 161
Ethoprophos 5.98 243 -> 131 243 -> 97
Ethoxyquin 7.4 218.1 -> 174 218.1 -> 160
Etoxazole 9 360.1 -> 141 360.1 -> 57.2
Famoxadone 7.72 392 -> 331 392 -> 238
Fenamidone 6.65 312.1 -> 92.1 312.1 -> 65
Fenamiphos 7.5 304.2 -> 217.1 304.2 -> 202.1
Fenarimol 7.3 331 -> 268 331 -> 81
Fenazaquin 9.91 307 -> 161 307 -> 147
Fenbuconazole 7.57 337 -> 125 337 -> 70
Fenhexamid 7.04 302 -> 97 302 -> 55
Fenitrothion 6.4 278 -> 125.2 278 -> 109
Fenoxycarb 7.53 302.2 -> 88.1 302.2 -> 116.2
Fenpropimorph 9.84 304 -> 147 304 -> 117
Fenpyroximate 9.29 422 -> 366.1 442 -> 135.1
Fenthion 6.9 278.9 -> 169 278.9 -> 246.9
Fenuron 3.85 165.1 -> 72.1 165.1 -> 46
Flonicamid 2.25 230.1 -> 203.1 230.1 -> 174
Flucarbazone 2.81 397.1 -> 130.1 397.1 -> 115
Flufenacet 7.17 364.1 -> 152.2 364.1 -> 194.2
Flufenoxuron 8.98 489.1 -> 158.2 489.1 -> 141.2
Fluometuron 5.98 233.1 -> 72.1 233.1 -> 46
Fluopicolid 7.2 385 -> 174.8 383 -> 173
Fluoxastrobin 7.34 459.2 -> 427.2 459.2 -> 188
Fluquinconazole 7.31 376 -> 349 376 -> 307
Flusilazole 7.6 316 -> 247 316 -> 165
Flutolanil 6.81 341.1 -> 242.1 341.1 -> 262.1
Flutriafol 5.99 302 -> 123 302 -> 109
Fonophos 5.9 247 -> 183 247 -> 201
Forchlorfenuron 6.37 248 -> 93.1 248 -> 165.1
Formetanate 4.48 222 -> 165.1 222 -> 120
Fuberidazole 5.5 185 -> 157 185 -> 65
Furalaxyl 6.62 302.1 -> 95.1 302.1 -> 242.1
Furathiocarb 8.42 383.2 -> 195.2 383.2 -> 252.2
Hexaconazole 7.95 314 -> 70 314 -> 159
Hexaflumuron 8.42 461.1 -> 158.2 461.1 -> 141.1
Hexythiazox 8.77 353 -> 228 353 -> 168
Hydramethylnon 9.2 495.2 -> 323.1 495.2 -> 151
Imazalil 8.1 297.1 -> 159.2 299.1 -> 161.2
Imazapyr 7.3 262.2 -> 217.2 262.2 -> 202.2
Imidacloprid 3.82 256.2 -> 209 256.2 -> 175.2
Indoxacarb 8.03 528 -> 203 528 -> 56
Ipconazole 8.33 334.2 -> 70 334.2 -> 125
Iprodion 7.6 330.1 -> 244.9 332.1 -> 247
Iprovalicarb 6.98 321.2 -> 119 321.2 -> 203.2
Irgarol 7.72 254.2 -> 198.1 254.2 -> 83.2
Isofenphos 6.66 314 -> 120 314 -> 162.1
Isoprocarb 5.87 194.2 -> 95.1 194.2 -> 137.2
Isoproturon 6.42 207.2 -> 72.1 207.2 -> 46.1
Isoxaflutole 5.87 360.1 -> 251.1 360.1 -> 220.1
Kresoxim-methyl 7.53 314 -> 116 314 -> 206
Lenacil 6.5 235.3 -> 153.2 235.3 -> 136.2
Linuron 6.99 249.1 -> 160 249.1 -> 182.1
Lufenuron 8.83 511.1 -> 158.1 511.1 -> 141.2
Malathion 6.83 331 -> 99.1 331 -> 127.1
Mandipropamid 6.81 412.1 -> 328.1 412.1 -> 355.9
Mefenacet 7.35 299 -> 148.1 299 -> 120.1
Mepanipyrim 7.57 224 -> 106 224 -> 77
Mepronil 6.86 270.1 -> 119.1 270.1 -> 228
Metalaxyl 6.13 280.2 -> 220.2 280.2 -> 192.3
Metconazole 8.01 320 -> 70 320 -> 125
Methabenzthiazuron 6.56 222.1 -> 165.2 222.1 -> 150.3
Methamidophos 1.06 142 -> 94 142 -> 125
Methiocarb 6.82 226.1 -> 169.2 226.1 -> 121.1
Methomyl 2.62 163.1 -> 88.1 163.1 -> 106
Methoprotryne 6.95 272.2 -> 240.2 272.2 -> 198
Methoxyfenozide 6.9 369 -> 149 369 -> 133
Metobromuron 6.22 259 -> 170.2 259 -> 148.2
Metolachlor 7.5 284.2 -> 252.2 284.2 -> 176.2
Metoxuron 5.1 229 -> 72.1 229 -> 156.1
Metribuzin 5.14 215.1 -> 187.2 215.1 -> 84.1
Mevinphos 4.29 225 -> 127 225 -> 193
Mexacarbate 7.02 223.2 -> 166.2 223.2 -> 151
Milbemectin A3 10.3 546.4 -> 511.3 546.4 -> 493.3
Milbemectin A4 10.5 560.4 -> 525.4 560.4 -> 55.2
Molinate 7.3 188.2 -> 126.2 188.2 -> 55.1
Monocrotophos 2.9 224 -> 127 224 -> 98
Monolinuron 5.93 215.1 -> 126.1 215.1 -> 99
Monuron 5.7 199.2 -> 72.2 199.2 -> 126.3
Myclobutanil 7.17 289 -> 70 289 -> 125
Neburon 7.65 275 -> 88 275 -> 114
Nitenpyram 2.55 271.2 -> 126.1 271.2 -> 237.2
Novaluron 8.38 493 -> 158.1 493 -> 141.1
Nuarimol 6.7 315 -> 252 315 -> 81
Omethoate 1.69 214 -> 124.9 214 -> 182.8
Oxadixyl 4.85 279.2 -> 219.2 279.2 -> 132.1
OxamyI 2.35 237.1 -> 72.1 237.1 -> 90.1
Oxydemeton-methyl 3.1 247 -> 169 247 -> 109
Paclobutrazol 6.82 294 -> 70 294 -> 125
Parathion-ethyl 6.7 292.1 -> 236.2 292.1 -> 94.1
Parathion-methyl 7.6 263.9 -> 232.1 263.9 -> 125
Penconazole 8.01 284 -> 159 284 -> 70
Pencycuron 8.1 329.1 -> 125.1 331.2 -> 127
Phenmedipham 6.35 301.1 -> 136 301.1 -> 168.1
Phenthoate 7 321 -> 163 321 -> 79
Phosmet 6.7 318 -> 160 318 -> 133
Phoxim 7.9 299.1 -> 129.1 299.1 -> 77.1
Picoxystrobin 7.44 368 -> 145 368 -> 205
Pinoxaden 7.99 401.3 -> 317.2 401.1 -> 57
Piperonyl butoxide 8.62 356.2 -> 177.2 356.2 -> 119
Pirimicarb 6.29 239.2 -> 72.1 239.2 -> 182.2
Pirimicarb-
desmethylformamido
6.4 253.2 -> 72.1 253.2 -> 225.3
Pirimiphos-ethyl 7.48 334 -> 198.1 334 -> 182.1
Prochloraz 8.29 376.1 -> 308 376.1 -> 70.1
Promecarb 6.86 208.2 -> 109.1 208.2 -> 151.1
Prometon 6.86 226.1 -> 142.1 226.1 -> 86
Prometryn 7.4 242.2 -> 200.1 242.2 -> 158.1
Propachlor 6.2 212.2 -> 170.1 212.2 -> 94.1
Propamocarb 6.61 189.2 -> 102.2 189.2 -> 73.9
Propargite 8.79 368 -> 231 368 -> 175
Propazine 6.9 230.1 -> 146.1 230.1 -> 188.1
Propham 5.78 180 -> 138 180 -> 120
Propiconazole 7.98 342.1 -> 159.1 342.1 -> 69.1
Propoxur 5.03 210.1 -> 111 210.1 -> 168.1
Prosulfocarb 8.5 252.3 -> 91.1 252.3 -> 128.1
py Cinerin I 9 317.2 -> 149 317.2 -> 106.9
py Cinerin II 8.2 361.2 -> 149 361.2 -> 106.9
Pymetrozin 3.61 218 -> 105 218 -> 78
Pyracarbolid 5.4 218.2 -> 125 218.2 -> 97
Pyraclostrobin 7.95 388 -> 194 388 -> 163
Pyrazophos 7.8 374 -> 222 374 -> 194
Pyrethrin I 8.9 329.2 -> 160.9 329.2 -> 132.9
Pyrethrin II 8.3 373.1 -> 160.9 373.1 -> 308.9
Pyridaben 9.33 365 -> 147 365 -> 309
Pyridaphenthion 7.8 341 -> 189 341 -> 205
Pyrimethanil 7.24 200 -> 107 200 -> 82
Pyriproxyfen 8.72 322 -> 96 322 -> 185
Quinalphos 6.7 299 -> 147 299 -> 163
Quinoxyfen 9.12 308 -> 197 308 -> 162
Rotenone 7.61 395 -> 213 395 -> 192
Secbumeton 6.85 226.2 -> 170.1 226.2 -> 100
Siduron 6.55 233.3 -> 137.2 233.3 -> 94
Simetryn 6.36 214 -> 124 214 -> 144
Spinosyn A 11.3 732.6 -> 142.1 732.6 -> 98
Spinosyn D 11.6 746.6 -> 142.1 746.6 -> 98
Spirodiclofen 8.96 411.3 -> 313.2 411.3 -> 213.1
Spiromesifen 8.8 371.3 -> 273 371.3 -> 255
Spiroxamine (isomer 1) 10.7; 11 298.4 -> 144.2 298.4 -> 100.2
Spiroxamine (isomer 2) 10.7; 11 298.3 -> 144.2 298.3 -> 100.2
Sulfentrazone 4.77 387 -> 307.1 387 -> 146
Sulfotep-ethyl 7 323 -> 115 323 -> 171.1
Sulprofos 7 323 -> 219 323 -> 247
Tebuconazole 7.8 308 -> 70 308 -> 125
Tebufenozide 7.39 353.1 -> 133.1 353.1 -> 297.1
Tebufenpyrad 8.56 334 -> 117 334 -> 145
Tebuthiuron 5.71 229.2 -> 172.4 229.2 -> 116.1
Teflubenzuron 8.81 381.1 -> 141.2 381.1 -> 158.2
Terbufos 6.5 289 -> 103 289 -> 57
Terbumeton 6.84 226 -> 170 226 -> 114
Terbutryn 7.57 242.2 -> 186.1 242.2 -> 68.1
Tetraconazole 7.3 372 -> 159 372 -> 70
Thiabendazole 5.71 202.1 -> 175.1 202.1 -> 131.2
Thiacloprid 4.89 253.1 -> 126.1 253.1 -> 99.1
Thiamethoxam 3.06 292 -> 211 292 -> 181
Thidiazuron 5.18 221.2 -> 102.1 221.2 -> 127.9
Thiobencarb 8.09 258.1 -> 125 258.1 -> 89
Thiofanox 5.7 219 -> 57.1 219 -> 60.9
Thiofanoxsulfon 3.4 251.1 -> 75.9 251.1 -> 57
Thiofanoxsulfoxid 3.6 235.1 -> 104.1 235.1 -> 57
Thiophanate-methyl 5.1 343 -> 151 343 -> 192
Tolclofos-methyl 8 301 -> 175 301 -> 268.9
Topramezone 1.73 364.1 -> 334.1 364.1 -> 125
Triadimefon 6.94 294 -> 197 294 -> 225
Triadimenol 7.04 296.1 -> 70.1 296.1 -> 227.2
Tricyclazole 5.18 190 -> 163 190 -> 136
Trifloxystrobin 8.09 409 -> 186 409 -> 206
Triflumizole 8.47 346 -> 278 346 -> 73
Triflumuron 7.94 359.1 -> 156.2 359.1 -> 139
Triticonazole 7.38 318 -> 70 318 -> 125
Uniconazole 7.4 292.2 -> 70.1 292.2 -> 43
Vamidothion 3.75 288 -> 146 288 -> 118
Zoxamide 7.7 336.1 -> 186.9 338.1 -> 188.7

Data for 3µm Ultra Aqueous C18 column are shown.

Table 2: Peak list for pesticides in negative ion mode.
Compound ID Retention Time
(min)
Transition 1 Transition 2
Acrinathrin 9.09 540 -> 372 540 -> 372
Chlorfluazuron 9.24 539.9 -> 356.8 539.9 -> 356.8
Clothianidin 3.63 249.9 -> 58 247.9 -> 59
Diflubenzuron 7.68 309 -> 156.1 310 -> 288.9
Diuron 6.78 230.9 -> 185.8 230.9 -> 149.8
Fluazinam 7.99 462.5 -> 415.8 462.5 -> 397.9
Fludioxonil 6.93 246.9 -> 179.9 246.9 -> 125.9
Forchlorfenuron 6.44 246 -> 126.9 246 -> 91.2
Hexaflumuron 8.45 459 -> 438.8 459 -> 174.9
Imibenconazol 8.82 409 -> 250.9 411 -> 253
Lufenuron 8.87 509 -> 325.9 509 -> 175
Metaflumizon 8.5 505.1 -> 301.9 505.1 -> 140.9
Metamitron 3.97 201 -> 184.8 201 -> 116.9
Methoxyfenozid 6.98 366.9 -> 104.9 366.9 -> 148.9
Nitenpyram 2.59 269.2 -> 221.6 269.2 -> 100.8
Novaluron 8.42 491.1 -> 470.7 493.1 -> 472.7
Profoxydim 7.57 464.3 -> 277.9 464.3 -> 126.8
Propoxycarbazon 3.09 396.9 -> 156 396.9 -> 112.9
Prothioconazol 7.16 342 -> 100.1 343.9 -> 99.9
Tebufenozid 7.46 350.9 -> 149 350.9 -> 105
Teflubenzuron 8.85 378.6 -> 338.8 378.6 -> 195.9
Tepraloxydim 4.57 340 -> 248 340 -> 220.1
Terbacil 5.38 214.9 -> 158.9 216.9 -> 160.9
Tralkoxydim 6.28 328.1 -> 253.8 328.1 -> 281.8
Triadimefon 7.01 292 -> 67.9 292 -> 234.9
Triflumuron 8 356.9 -> 153.8 358.9 -> 155.9

Data for 3µm Ultra Aqueous C18 column are shown.

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